LYN antibody (N-Term)

Catalog No. ABIN233331

This anti-LYN antibody is a Rabbit Polyclonal antibody detecting LYN in WB, ELISA, IHC, IP and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for LYN antibody (N-Term) (ABIN233331)

Target: LYN (V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LYN antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-LYN Antibody

Binding Specificity: N-Term

Specificity: Detects endogenous levels of the 56kD isoform of Lyn. It does not recognize the 53kD Lyn isoform. Species cross-reactivity: Mouse and rat.

Purification: Protein A purified

Immunogen: Synthetic peptide (KLH coupled) derived from the amino-terminal sequence of human Lyn.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P, IP (1:100), WB (1:1000)

Usage: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 1:1000, incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:100. Immunohistochemistry (Paraffin): 1:50.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for LYN

Target: LYN (V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN))

Alternative Name: LYN

Background: Name/Gene ID: LYN
Subfamily: Src
Family: Protein Kinase

Synonyms: LYN, Lyn protein tyrosine kinase, Lyn ptk, Lyn tyrosine kinase, p56Lyn, p53Lyn, Tyrosine-protein kinase Lyn, JTK8, Lyn kinase

Gene ID: 4067

UniProt: P07948

Pathways: Fc-epsilon Receptor Signaling Pathway, Hormone Transport, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling, Integrin Complex, BCR Signaling