MMP2 antibody (APMA-Activated)

Catalog No. ABIN233363

The Mouse Monoclonal anti-MMP2 antibody has been validated for IHC, IHC (p) and IHC (fro). It is suitable to detect MMP2 in samples from Human.

Quick Overview for MMP2 antibody (APMA-Activated) (ABIN233363)

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MMP2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-MMP2 Antibody

Binding Specificity: APMA-Activated

Specificity: Recognizes proteins of 72kD and ~66kD which are identified as pro (latent) and active forms of matrix metalloproteinase-2 (MMP-2, also known as 72kD collagenase IV or gelatinase A). No cross-reaction with pro and active forms of other MMPs.

Purification: Protein G purified

Immunogen: Human APMA-activated native 72kD Gelatinase A.

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-Fr (2 - 4 μg/mL), IHC-P (2 - 4 μg/mL)

Usage: Immunohistochemistry (Frozen & fixed-paraffin embedded) 2-4 μg/mL g/mL 30 min at RT. Staining of formalin-fixed tissues is improved by boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: Conditioned, serum-free medium from (dexamethasone-treated) human fibrosarcoma HT-1080 or endothelial HUVEC cells. Placenta, bladder, breast and ovarian carcinomas. S.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM PBS,  pH 7.4, 0.2 % BSA, 15 mM sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for MMP2

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Alternative Name: MMP2 / MMP-2

Background: Name/Gene ID: MMP2
Subfamily: Metallopeptidase M10A
Family: Protease

Synonyms: MMP2, 72 kd type iv collagenase, 72 kDa gelatinase, 72 kd gelatinase, 72 kDa type IV collagenase, CLG4A, CLG4, GelA, Gelatinase A, Matrix metalloproteinase-2, MMP-2, MONA, Neutrophil gelatinase, TBE-1, Tbe-1 metalloprotease, Collagenase type IV-A, Matrix metalloproteinase-II, MMP-II

Gene ID: 4313

UniProt: P08253

Pathways: Activation of Innate immune Response