SUMO antibody

Catalog No. ABIN233746

Product Details anti-SUMO Antibody

Target: SUMO

Reactivity: Saccharomyces cerevisiae

Host: Mouse

Clonality: Monoclonal

Conjugate: This SUMO antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This antibody was produced in mice by repeated immunizations with full-length recombinant yeast SUMO protein.
Immunogentype:Recombinant

Clone: 4F2-F5-G2

Isotype: IgG

Application Details

Application Notes: This monoclonal antibody reacts with yeast SUMO (Smt3) by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.  Using the specified conditions, this antibody may recognize other prominent intrinsic bands (UBLs or conjugates).  Other intrinsic bands are readily detectable at lower dilutions.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.9 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-SUMO antibody (ABIN233746)

Qian, Bradford, Cooke, Lyons: "Grb7 and Hax1 may colocalize partially to mitochondria in EGF-treated SKBR3 cells and their interaction can affect Caspase3 cleavage of Hax1." in: Journal of molecular recognition : JMR, Vol. 29, Issue 7, pp. 318-33, (2017) (PubMed).

Meyer, Shah, Zhang, Rohrs, Rao: "Evidence for intermolecular interactions between the intracellular domains of the arabidopsis receptor-like kinase ACR4, its homologs and the Wox5 transcription factor." in: PLoS ONE, Vol. 10, Issue 3, pp. e0118861, (2016) (PubMed).

Target Details for SUMO

Target: SUMO

Abstract: SUMO Products

Synonyms: CG4494 antibody, Dm0342 antibody, DmSUMO-1 antibody, DmSmt3 antibody, Dmel\\CG4494 antibody, Dmsmt3 antibody, SMT3 antibody, SUMO antibody, Smt3 antibody, Sumo antibody, anon-EST:Posey240 antibody, dSmt3 antibody, dsmt3 antibody, l(2)04493 antibody, l(2)SH0182 antibody, l(2)SH2 0182 antibody, sumo antibody, CG4494 gene product from transcript CG4494-RA antibody, smt3 antibody

Background: Covalent modification of cellular proteins by the ubiquitin-like modifier SUMO (small ubiquitin-like modifier) regulates various cellular processes, such as nuclear transport, signal transduction, stress responses and cell cycle progression.  However, in contrast to ubiquination, sumoylation does not tag proteins for degradation by the 26S proteasome but rather seems to enhance stability or modulate their subcellular compartmentalization.  Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin.  Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12.  Proteins of the second class, including parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs).  These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other.  In contrast to UBLs, UDPs are not conjugated to other proteins.  Once covalently attached to cellular targets, SUMO regulates protein:protein and protein:DNA interactions, as well as localization and stability of the target protein.  Sumoylation occurs in most eukaryotic systems, and SUMO is highly conserved from yeast to humans.   Where invertebrates have only a single SUMO gene termed SMT3, three members
Synonyms: Ubiquitin-like protein SMT3 antibody, SMT3 antibody

Gene ID: 852122, 6320718