RAB11FIP3 antibody (C-Term)

Catalog No. ABIN233825

Product Details anti-RAB11FIP3 Antibody

Target: RAB11FIP3 (RAB11 Family Interacting Protein 3 (Class II) (RAB11FIP3))

Binding Specificity: C-Term

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAB11FIP3 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids spanning the one-third of human FIP3.
Immunogentype:Recombinant

Isotype: IgG

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Although the predicted molecular weight of FIP3 is 82.4 kDa, a band corresponding to FIP3 was observed at 120 kDa in human cell lysates and at 150 kDa in mouse cell lysates.  The identity of a 40 kDa band, observed in western blotting against both HeLa and 293T cell lysates, is unknown (personal communication, P. Randazzo, CCR-NCI, Bethesda, MD).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.19 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for RAB11FIP3

Target: RAB11FIP3 (RAB11 Family Interacting Protein 3 (Class II) (RAB11FIP3))

Abstract: RAB11FIP3 Products

Synonyms: LRRGT00043 antibody, RGD1308952 antibody, MNDPK antibody, RAB11FIP3 antibody, CART1 antibody, Rab11-FIP3 antibody, Cart1 antibody, D030060O14Rik antibody, mKIAA0665 antibody, si:ch211-153c20.4 antibody, zC153C20.4 antibody, RAB11 family interacting protein 3 antibody, NME/NM23 nucleoside diphosphate kinase 4 antibody, RAB11 family interacting protein 3 (class II) antibody, Rab11fip3 antibody, NME4 antibody, RAB11FIP3 antibody, rab11fip3 antibody

Background: Rab11 Family-Interacting Protein 3 (FIP3)/Arfophilin-1 and FIP4/Arfophilin-2 are dual effectors for Rab11 and the ADP-ribosylation factor Arf6.  Rab11 and Arf6 are involved in membrane delivery from recycling endosomes to the plasma membrane during cytokinesis.  The dual Rab11/Arf6 binding proteins, FIP3 and FIP4, function in the delivery of recycling endosomes to the cleavage furrow and are, together with Rab11, essential for completion of abscission, the terminal step of cytokinesis.  An interaction between Rab11-FIP3 and ASAP1 has also been identified (personal communication, P. Randazzo, CCR-NCI, Bethesda, MD).  ASAP1 is an Arf GTPase-activating protein that has been implicated in the invasive properties of uveal melanoma and mammary carcinoma.
Synonyms: IkB kinase associated protein 1 antibody, IkB kinase subunit gamma antibody, Inhibitor of nuclear factor kappa B kinase subunit gamma antibody, AMCBX1 antibody, FIP 3 antibody

Gene ID: 9727, 7662234

UniProt: O75154