THRA antibody (N-Term)

Catalog No. ABIN233838

Product Details anti-THRA Antibody

Target: THRA (Thyroid Hormone Receptor, alpha (THRA))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This THRA antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human THRA protein.  A BLAST analysis was used to suggest cross-reactivity with THRA from mouse, human and rat based on a 100% homology with the immunizing sequence.  Cross-reactivity with THRA from other sources has not been determined.

Characteristics: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Thyroid hormone receptor alpha is a nuclear hormone receptor with high affinity for the hormone triiodo-thyronine. THRA is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having a certain extent of redundancy, may mediate different functions of thyroid hormone. THRA interacts with NCOA3 and NCOA6 co-activators, leading to a strong increase in transcription of target genes. THRA is localized within the nucleus and has been found to exist as 4 isoforms originating from alternative splicing variants. This antibody recognizes THRA isoform 1. Isoform 1 has a distinct C-terminus compared to isoform 2.

Purification: affinity purified

Sterility: Sterile filtered

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of human THRA isoform 1 protein.
Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA and western blotting.

Comment:

Gene Name: THRA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.89 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is three (3) months from date of opening.

Expiry Date: 3 months

References for anti-THRA antibody (ABIN233838)

Contreras-Jurado, García-Serrano, Martínez-Fernández, Ruiz-Llorente, Paramio, Aranda: "Impaired hair growth and wound healing in mice lacking thyroid hormone receptors." in: PLoS ONE, Vol. 9, Issue 9, pp. e108137, (2015) (PubMed).

Mishra, Zhu, Ge, Cheng: "Adipogenesis is differentially impaired by thyroid hormone receptor mutant isoforms." in: Journal of molecular endocrinology, Vol. 44, Issue 4, pp. 247-55, (2010) (PubMed).

Target Details for THRA

Target: THRA (Thyroid Hormone Receptor, alpha (THRA))

Alternative Name: THRA (THRA Products)

Synonyms: AR7 antibody, CHNG6 antibody, EAR7 antibody, ERB-T-1 antibody, ERBA antibody, ERBA1 antibody, NR1A1 antibody, THRA1 antibody, THRA2 antibody, c-ERBA-1 antibody, C-ERBA-ALPHA antibody, TRalpha antibody, c-erba antibody, erba antibody, nr1a1-A antibody, thra-A antibody, thra1 antibody, TR[a]1 antibody, nr1a1 antibody, thra antibody, thral antibody, tra antibody, tra1 antibody, wu:fb59g07 antibody, Thra1 antibody, 6430529J03Rik antibody, AW259572 antibody, Erba antibody, Nr1a1 antibody, Rvr antibody, T3R[a] antibody, T3Ralpha antibody, Thra2 antibody, C-ERBA ALPHA1 antibody, Thra antibody, fTHRalphaA antibody, nr1a1-a antibody, thyroid hormone receptor, alpha antibody, thyroid hormone receptor, alpha L homeolog antibody, thyroid hormone receptor alpha a antibody, thyroid hormone receptor alpha antibody, thyroid hormone receptor alpha-A antibody, THRA antibody, thra.L antibody, thraa antibody, Thra antibody, tr-alpha antibody, LOC109641414 antibody

Background: This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. Thyroid hormone receptor alpha is a nuclear hormone receptor with high affinity for the hormone triiodo-thyronine.  THRA is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having a certain extent of redundancy, may mediate different functions of thyroid hormone.  THRA interacts with NCOA3 and NCOA6 co-activators, leading to a strong increase in transcription of target genes. THRA is localized within the nucleus and has been found to exist as 4 isoforms originating from alternative splicing variants.  Isoform 1 has a distinct C-terminus compared to isoform 2.
Synonyms: Thyroid hormone receptor alpha Nuclear receptor subfamily 1 group A member 1 c-erbA-alpha c-erbA-1 V-erbA-related protein 7 EAR-7

Gene ID: 7067, 40806160

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Sensory Perception of Sound, Cellular Response to Molecule of Bacterial Origin, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Maintenance of Protein Location, Skeletal Muscle Fiber Development