SMAD2 antibody (Internal Region)

Catalog No. ABIN233848

Product Details anti-SMAD2 Antibody

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: This affinity purified antibody is directed against human Smad2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.  A BLAST analysis was used to suggest cross-reactivity with Smad2 protein from human, mouse and rat based on 100% homology with the immunizing sequence.  Reactivity against homologues from other sources is not known.  Also, the antibody is Smad2 specific, and reactivity to other Smad proteins (specifically Smad1, Smad3, Smad4, and Smad7) is not detected in over-expressed cell lysates (Personal Communication, Kathleen Flanders, CCR-NCI, Bethesda, MD).

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Characteristics: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Smad2 (also known as Mothers against decapentaplegic homolog 2, Mothers against DPP homolog 2, Mad2, hMAD-2 or hSMAD2) is a member of the Smad family of proteins which are similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the

Purification: affinity purified

Sterility: Sterile filtered

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Smad23 protein.
Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 52 kDa in size corresponding to Smad2 protein by western blotting in the appropriate cell lysate or extract.

Comment:

Gene Name: SMAD2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.55 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is three (3) months from date of opening.

Expiry Date: 3 months

References for anti-SMAD2 antibody (ABIN233848)

Chung, Overstreet, Li, Wang, Niu, Wang, Fan, Sasaki, Jin, Khodo, Gewin, Zhang, Harris: "TGF-β promotes fibrosis after severe acute kidney injury by enhancing renal macrophage infiltration." in: JCI insight, Vol. 3, Issue 21, (2018) (PubMed).

Target Details for SMAD2

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Alternative Name: SMAD2 (SMAD2 Products)

Synonyms: JV18 antibody, JV18-1 antibody, MADH2 antibody, MADR2 antibody, hMAD-2 antibody, hSMAD2 antibody, jv18 antibody, Xmad2 antibody, madh2 antibody, madr2 antibody, XSmad2 antibody, hmad-2 antibody, hsmad2 antibody, jv18-1 antibody, smad-2 antibody, fj43c06 antibody, wu:fj43c06 antibody, 7120426M23Rik antibody, Madh2 antibody, Madr2 antibody, Smad-2 antibody, mMad2 antibody, MAD homolog 2 antibody, Mothers against DPP homolog 2 antibody, SMAD 2 antibody, Smad2 antibody, SMAD family member 2 antibody, SMAD family member 2-Z antibody, SMAD family member 2 S homeolog antibody, SMAD2 antibody, SMAD2Z antibody, smad2 antibody, smad2.S antibody, Smad2 antibody

Background: This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. Smad2 (also known as Mothers against decapentaplegic homolog 2, Mothers against DPP homolog 2, Mad2, hMAD-2 or hSMAD2) is a member of the Smad family of proteins which are similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the
Synonyms: MAD antibody, MAD Related Protein 2 antibody, MGC34440 antibody, Mothers Against Decapentaplegic Homolog 2 antibody

Gene ID: 4087, 5174511

UniProt: Q15796

Pathways: Cell Division Cycle, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus