ACRV1 antibody

Catalog No. ABIN238420

This Mouse Monoclonal antibody specifically detects ACRV1 in WB, IF and FACS. It exhibits reactivity toward Human and Mouse.

Quick Overview for ACRV1 antibody (ABIN238420)

Target: ACRV1 (Acrosomal Vesicle Protein 1 (ACRV1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This ACRV1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: Hs-14

Product Details anti-ACRV1 Antibody

Specificity: The antibody Hs-14 reacts with a 220 kDa testis-specific intra-acrosomal protein associated with the membranes of the acrosomal vesicle.

Characteristics: Synonyms: Acrosomal protein SP-10, SP10, SPACA2, Acrosomal vesicle protein 1, Intra-acrosomalSperm Protein

Purification: Precipitation methods and size-exclusion chromatography

Purity: > 95 % (by SDS-PAGE)

Immunogen: Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid,10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0. 2% aceticacid and subsequently used for immunization.

Isotype: IgM

Application Details

Application Notes: Flow Cytometry. Western Blotting. Immunocytochemistry: 10 μg/mL, Staining technique: Membrane permeabilization(acetone) is essential. Application note: The antibody Hs-14 is designed for quantitativeimmunofluorescence analysis of pathological sperms (excellent tool for laboratories ofassisted reproduction when optimal method of fertilization is sought).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody at 2 - 8 °C. DO NOT FREEZE!
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for ACRV1

Target: ACRV1 (Acrosomal Vesicle Protein 1 (ACRV1))

Alternative Name: ACRV1

Background: One of the most frequent causes of man infertility is defective sperm acrosome. This damage can be detected using antibodies against intra-acrosomal proteins. Besides diagnostics of sperm pathology, monoclonal antibodies against intra-acrosomal proteins can be used for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.Synonyms: Acrosomal protein SP-10, Acrosomal vesicle protein 1, Intra-acrosomal Sperm Protein, SP10, SPACA2

Gene ID: 56

UniProt: P26436