PAM antibody (C-Term)

Catalog No. ABIN238682

The Goat Polyclonal anti-PAM antibody has been validated for WB and ELISA. It is suitable to detect PAM in samples from Human.

Quick Overview for PAM antibody (C-Term) (ABIN238682)

Target: PAM (Peptidylglycine alpha-Amidating Monooxygenase (PAM))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PAM antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-PAM Antibody

Binding Specificity: C-Term

Purpose: PAM / PAL

Sequence: DQEKEDDGSE SEE

Specificity: This antibody is expected to recognise all reported isoforms (NP_000910.2, NP_620121.1, NP_620176.1 and NP_620177.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-DQEKEDDGSESEE

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 100 kDa band observed in Human Kidney lysates (calculated MW of 101 kDa according to preproprotein NP_620121.1). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:4000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PAM

Target: PAM (Peptidylglycine alpha-Amidating Monooxygenase (PAM))

Alternative Name: PAM

Background: PAM, PAL, peptidylglycine alpha-amidating monooxygenase , PHM, pancreatic peptidylglycine alpha-amidating monooxygenase, peptidyl alpha-amidating enzyme, peptidyl-alpha-hydroxyglycine alpha-amidating lyase, peptidylglycine 2-hydroxylase, peptidylglycine a

Gene ID: 5066

NCBI Accession: NP_000910, NP_620121, NP_620176, NP_620177