VCP antibody

Catalog No. ABIN239859

The Mouse Monoclonal anti-VCP antibody (Clone 4G9) (ABIN239859) specifically detects VCP in WB, IHC, IF, IP and IHC (p). The antibody is reactive with Human samples.

Quick Overview for VCP antibody (ABIN239859)

Target: VCP (Valosin Containing Protein (VCP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This VCP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 4G9

Product Details anti-VCP Antibody

Specificity: Recombinant human valosin containing protein

Purification: Affinity purified

Immunogen: Recombinant human VCP.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: IF (1 - 4 μg/mL), IHC, IHC-P (5 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Phosphate-buffered solution,  pH 7.2, 0.09 % sodium azide at 0.5 mg/mL. Sourced in TCS.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C

Storage Comment: Store at 4°C. Do not freeze.

Target Details for VCP

Target: VCP (Valosin Containing Protein (VCP))

Alternative Name: VCP

Background: Name/Gene ID: VCP

Synonyms: VCP, 15S Mg(2+)-ATPase p97 subunit, ALS14, TER ATPase, TERA, Valosin containing protein, Yeast Cdc48p homolog, Valosin-containing protein, IBMPFD, p97

Gene ID: 7415

UniProt: P55072

Pathways: ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Ubiquitin Proteasome Pathway