His Tag antibody (C-Term, N-Term)

Catalog No. ABIN2443717

This Mouse Monoclonal antibody specifically detects His Tag in WB, ELISA, IP, IHC and DB. It has been mentioned in 1 publication

Quick Overview for His Tag antibody (C-Term, N-Term) (ABIN2443717)

Target: His Tag

Host: Mouse

Clonality: Monoclonal

Conjugate: This His Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Dot Blot (DB)

Clone: HIS-H8

Product Details anti-His Tag Antibody

Binding Specificity: C-Term, N-Term

Purpose: His Tag Mouse Monoclonal Antibody (HIS.H8)

Sequence: HHHHHH

Specificity: The antibody recognizes His-tag encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e. reacts with N-terminal, C-terminal or internal 6x His-tags)

Purification: Protein G purified

Immunogen: The antibody was raised against HHHHHH (6x His) synthetic peptide conjugated to KLH.

Isotype: IgG2b

Application Details

Application Notes: WB 1:1000,IHC 1:500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 100 μL: WB 1:1000-3,000, IHC 1:500-2,000. Optimal dilution has to be determined by the user.

Buffer: Lyophilized protein G purified in PBS pH 7.4

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

References for anti-His Tag antibody (ABIN2443717)

Bouvette, Korkut, Fouillen, Amellah, Nanci, Durocher, Omichinski, Legault: "High-yield production of human Dicer by transfection of human HEK293-EBNA1 cells grown in suspension." in: BMC biotechnology, Vol. 18, Issue 1, pp. 76, (2019) (PubMed).

Target Details for His Tag

Target: His Tag

Target Type: Tag

Background: Epitope tags have applications in the labeling, isolation and detection of proteins using immunoblotting, Immunoprecipitation and immunostaining techniques. Epitope tags can be used, by affinity chromatography, to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. Due its small size, the protein's biochemical properties seem not to be affected by the tag protein. Polyhistidine-tag is also known as histidine-tag, 6xHis-tag and by the trademarked name His-tag.