LDLR antibody (C-Term)

Catalog No. ABIN2443758

The Rabbit Polyclonal anti-LDLR antibody (ABIN2443758) specifically detects LDLR in WB. The antibody is reactive with Rat samples.

Quick Overview for LDLR antibody (C-Term) (ABIN2443758)

Target: LDLR (Low Density Lipoprotein Receptor (LDLR))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LDLR antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-LDLR Antibody

Binding Specificity: C-Term

Purpose: Low density lipoprotein receptor LDLR Rabbit Polyclonal Antibody

Specificity: Specifically recognizes the LDL receptor at a molecular weight of 160 kDa. The antibody recognizes the C-terminal portion of LDLR.

Immunogen: C-terminal peptide

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 100 μL: WB: 1:1500. Optimal dilution has to be determined by the user.

Buffer: Lyophilized serum

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for LDLR

Target: LDLR (Low Density Lipoprotein Receptor (LDLR))

Alternative Name: Low density lipoprotein receptor LDLR

Background:

LDL receptor

The LDL Receptor (LDLR) is a transmembrane protein of 839 amino acids that mediates the endocytosis of cholesterol-rich LDL. The LDL receptor plays a critical role in cholesterol homeostasis. It binds, internalizes, and removes LDL from serum. LDLR recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles, as well as the apoE protein found in chylomicron remnants and VLDL remnants (IDL). To be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits (or buds) on the cell surface. Defects in LDLR are the cause of familial hypercholesterolemia, a common autosomal semi-dominant disease that affects about 1 in 500 individuals.

UniProt: P35952

Pathways: Hepatitis C, Lipid Metabolism