APP antibody (cleaved, ISEVKMD Motif)

Catalog No. ABIN2443769

The Rabbit Polyclonal anti-APP antibody is suitable to detect APP in samples from Human. It has been validated for WB.

Quick Overview for APP antibody (cleaved, ISEVKMD Motif) (ABIN2443769)

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APP antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-APP Antibody

Binding Specificity: cleaved, ISEVKMD Motif

Purpose: Amyloid precursor protein cleaved by caspase-6 Rabbit Polyclonal Antibody

Specificity: The antibody recognizes the sequence (ISEVKMD) in Amyloid precursor protein (APP).

Immunogen: ISEVKMD peptide

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 50 μL: WB 1: 5000. Optimal dilution has to be determined by the user.

Buffer: Lyophilized serum

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for APP

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Alternative Name: Amyloid precursor protein

Background: Amyloid precursor protein (APP) functions as a cell surface receptor in the synapses of neurons and is implicated in neurite growth, neuronal adhesion, axonogenesis and cell mobility. APP is known as the precursor molecule whose proteolysis generates beta amyloid. Defects in APP are the cause of Alzheimer's disease type 1 and of amyloidosis cerebroarterial. Caspase activation has been found to have a central role in neuronal death and chronic neurodegenerations such as Huntington's, Parkinson's and Alzheimer's diseases. Proteolytic cleavage of specific substrates has also been demonstrated to be an important cellular event in the pathogenesis of AD and inhibition of cleavage of APP by caspase-6 was demonstrated to suppress synapse loss, dentate gyral atrophy, and memory loss.

UniProt: P10636

Pathways: Caspase Cascade in Apoptosis, EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, Feeding Behaviour