SLC17A7 antibody

Catalog No. ABIN2443817

The Mouse Monoclonal anti-SLC17A7 antibody is suitable to detect SLC17A7 in samples from Rat and Human. It has been validated for WB, IHC, ELISA and ICC.

Quick Overview for SLC17A7 antibody (ABIN2443817)

Target: SLC17A7 (Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7))

Reactivity: Rat, Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SLC17A7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)

Clone: McKA1

Product Details anti-SLC17A7 Antibody

Purpose: Vglut1 Mouse Monoclonal Antibody (McKA1)

Specificity: A highly specific and sensitive antibody for VGlut1.The antibody reveals the presence of the 58 kDa VGlut 1 protein on western blot of homogenized cerebral cortex cells lysates. It shows very precise reaction with VGlut1 epitope of human mid-frontal gyrus brain tissue in immunohistochemistry.

Purification: Protein G purified

Immunogen: Full Lenght VGlut1

Isotype: IgG2a

Application Details

Application Notes: ICC / IHC 1:5,IHC 1:5

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 100 μL: ICC / IHC 1:5,000. Optimal dilution has to be determined by the user

Buffer: Lyophilized protein G purified in PBS pH 7.4

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for SLC17A7

Target: SLC17A7 (Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7))

Alternative Name: Vglut1

Background:

Vesicular glutamate transporter 1, VGluT1, Brain-specific Na(+)-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 7

Glutamate is the major excitatory neurotransmitter in the central nervous system of mammals. Vesicular glutamate transporters (VGluts) are responsible for glutamate transport from the brain into native synaptic vesicles. The level of expression of the three known transporters in the adult brain determines the amount of glutamate that is loaded into vesicles and released.

UniProt: Q9P2U7