HGS antibody (full length)

Catalog No. ABIN2443821

The Mouse Monoclonal anti-HGS antibody (Clone 19-5) (ABIN2443821) specifically detects HGS in WB, IHC, ICC and IP. The antibody is reactive with Human, Mouse, Rat and Monkey samples.

Quick Overview for HGS antibody (full length) (ABIN2443821)

Target: HGS (Hepatocyte Growth Factor-Regulated tyrosine Kinase Substrate (HGS))

Reactivity: Human, Mouse, Rat, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This HGS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: 19-5

Product Details anti-HGS Antibody

Binding Specificity: full length

Purpose: Hrs (HGS) Mouse Monoclonal Antibody (19-5)

Specificity: The antibody recognizes an epitope is located between the VHS and coiled-coil domain of the HRS protein

Purification: Protein G purified

Immunogen: Full length hrs-2 fusion protein

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 50 μL: IHC: 1:300, ICC: 1:200, WB: 1:1000. Optimal dilution has to be determined by the user.

Buffer: Lyophilized protein G purified in PBS pH 7.4

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for HGS

Target: HGS (Hepatocyte Growth Factor-Regulated tyrosine Kinase Substrate (HGS))

Alternative Name: Hrs (HGS)

Background:

Hepatocyte growth factor-regulated tyrosine kinase substrate, HGS, Protein pp110

Hepatocyte growth factor-regulated tyrosine kinase substrate (HRS, also known as HGS) is a mammalian protein predominantly localized on early endosomes. Hrs interacts with many proteins involved in membrane trafficking, such as eps15, SNX-1 and SNAP-25. Hrs is implicated in early endosome fusion and may play a role in cargo sorting/trafficking to early and late endosome by recruiting clathrin. Hrs is also involved in intracellular signal transduction mediated by cytokines and growth factors.

UniProt: O14964

Pathways: EGFR Signaling Pathway, CXCR4-mediated Signaling Events, Synaptic Vesicle Exocytosis, EGFR Downregulation