SULT1E1 antibody (full length)

Catalog No. ABIN2443831

This Rabbit Polyclonal antibody specifically detects SULT1E1 in WB, IHC and IF. It exhibits reactivity toward Cow.

Quick Overview for SULT1E1 antibody (full length) (ABIN2443831)

Target: SULT1E1 (Sulfotransferase Family 1E Member 1 (SULT1E1))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SULT1E1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-SULT1E1 Antibody

Binding Specificity: full length

Purpose: Estrogen Sulfotransferase (EST) Rabbit Polyclonal Antibody

Specificity: A highly specific and sensitive antibody against the bovine Estrogen Sulfotransferase.

Purification: Immunogen affinity purified

Immunogen: Full length recombinant bovine EST protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 100μL: WB 1 μg/mL, IHC 5 μg/mL, IF 10 μg/mL. Optimal dilution has to be determined by the user.

Buffer: Lyophilized immunogen affinity purified in PBS pH 7.4

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for SULT1E1

Target: SULT1E1 (Sulfotransferase Family 1E Member 1 (SULT1E1))

Alternative Name: Estrogen Sulfotransferase (EST)

Background:

anti-estrogen sulfotransferase, anti-est , Sulfotransferase 1E1 , ST1E1 , Sulfotransferase, estrogen-preferring , SULT1E1

Estrogen sulfotransferase (EST) is a cytosolic enzyme that catalyzes specific sulfonation with a high affinity for estrogens. Because EST is involved in estrone and estradiol metabolism, its expression in the male reproductive tract determines where, along the tract, an estrogenic environment predominates. EST may also be involved in sperm physiology during the epididymal transit.

UniProt: P19217

Pathways: Steroid Hormone Biosynthesis