RBBP6 antibody

Catalog No. ABIN2443899

The Rabbit Polyclonal anti-RBBP6 antibody (ABIN2443899) specifically detects RBBP6 in WB, IF and IP. The antibody is reactive with Human and Mouse samples.

Quick Overview for RBBP6 antibody (ABIN2443899)

Target: RBBP6 (Retinoblastoma Binding Protein 6 (RBBP6))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RBBP6 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-RBBP6 Antibody

Purpose: PACT Rabbit Polyclonal Antibody

Specificity: The antibody recognizes the human PACT and the mouse homologous RAX.

Purification: Immunogen affinity purified

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 50 μL: IP: 10 μg, WB: 1:1000 - 1:3000, IF: 1:500. Optimal dilution has to be determined by the user.

Buffer: Lyophilized immunogen affinity purified in PBS pH 7.4

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for RBBP6

Target: RBBP6 (Retinoblastoma Binding Protein 6 (RBBP6))

Alternative Name: PACT

Background:

Interferon-inducible double stranded RNA-dependent protein kinase activator A

The Interferon-inducible double stranded RNA-dependent protein kinase activator, also known as PKR-associated protein X (RAX in mouse) or PACT (in human) is a cellular protein that heterodimerizes with PKR through its double-stranded RNA binding proteins (dsRBDs). PKR is an interferon (IFN)-induced serine-threonine protein kinase that plays a central role in IFN's antiviral and antiproliferative activities. In the absence of viral infections, PACT activates PKR in response to diverse stress signals as serum starvation and treatments with arsenite or peroxide, resulting in the induction of the apoptotic cascade. PACT is expressed in most cell types at a very low abundance, and overexpression of PACT causes PKR activation, leading to eIF2α phosphorylation. PACT has been shown to interact with another DRBM-containing protein, TRBP (TAR RNA binding protein), and form a triple complex with Dicer to facilitate the production of siRNA.

UniProt: O75569

Pathways: Regulatory RNA Pathways