ITK antibody (AA 1-26)

Catalog No. ABIN2443927

This Mouse Monoclonal antibody specifically detects ITK in WB, IP and EAA. It exhibits reactivity toward Human and Rat.

Quick Overview for ITK antibody (AA 1-26) (ABIN2443927)

Target: ITK (IL2-Inducible T-Cell Kinase (ITK))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITK antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Activity Assay (EAA)

Clone: 2F12

Product Details anti-ITK Antibody

Binding Specificity: AA 1-26

Purpose: Itk (IL2-inducible T-cell kinase) Mouse Monoclonal Antibody (2F12)

Specificity: A highly specific and sensitive antibody against Itk. The antibody recognizes both native and denatured Itk (amino acids 1-26). No detectable cross reactivity with other Itk family members such as Btk or Tec. S

Purification: Protein G purified

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 100μL: WB 2 μg/mL. Optimal dilution has to be determined by the user.

Buffer: Lyophilized protein G purified in PBS pH 7.4

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for ITK

Target: ITK (IL2-Inducible T-Cell Kinase (ITK))

Alternative Name: Itk (IL2-inducible T-cell kinase)

Background: IL2-inducible T-cell kinase (Itk) is located in cell membrane of T-cell lines and natural killer cell lines and plays a role in T-cell proliferation and differentiation. Defects in Itk are the cause of lymphoproliferative syndrome EBV-associated autosomal type 1 (LPSA1).

UniProt: Q08881

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway