UNC13D antibody (N-Term)

Catalog No. ABIN2443930

This Rabbit Polyclonal antibody specifically detects UNC13D in WB and IP. It exhibits reactivity toward Human.

Quick Overview for UNC13D antibody (N-Term) (ABIN2443930)

Target: UNC13D (Unc-13 Homolog D (UNC13D))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UNC13D antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-UNC13D Antibody

Binding Specificity: AA 1-273, N-Term

Purpose: Munc13-4 Rabbit Polyclonal Antibody

Specificity: The antibody recognizes the N-terminal of the human Munc13-4 protein (aa 1-273).

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 50 μL: WB: 1:2000, IP: native conditions. Optimal dilution has to be determined by the user.

Buffer: Lyophilized serum

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for UNC13D

Target: UNC13D (Unc-13 Homolog D (UNC13D))

Alternative Name: Munc13-4

Background:

Protein unc-13 homolog D, hMunc13-4, UNC13D, FHL3, HLH3, HPLH3, Munc13-4, unc-13 homolog D

Protein unc-13 homolog D (Munc13-4) is involved in cytotoxic granule exocytosis in lymphocytes. It regulates the assembly of recycling of late endosomal structures which leads to the formation of an endosomal exocytic compartment. It also regulates Ca2+-dependent secretory lysosome exocytosis in mast cells. Munc13-4 is highly expressed in the spleen, thymus and leukocytes and to a lesser extent in the lung and placenta, brain, heart, skeletal muscle and kidney. Defects in Munc13-4 are the cause of familial HL type 3 (FHL3).

UniProt: Q70J99

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process