NAGPA antibody (N-Term)

Catalog No. ABIN2444545

This Rabbit Polyclonal antibody specifically detects NAGPA in WB. It exhibits reactivity toward Human, Cow, Dog, Guinea Pig, Horse, Mouse, Rat and Zebrafish (Danio rerio).

Quick Overview for NAGPA antibody (N-Term) (ABIN2444545)

Target: NAGPA (N-Acetylglucosamine-1-phosphodiester alpha-N-Acetylglucosaminidase (NAGPA))

Reactivity: Human, Cow, Dog, Guinea Pig, Horse, Mouse, Rat, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NAGPA antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NAGPA Antibody

Binding Specificity: N-Term

Purpose: NAGPA Antibody - N-terminal region

Sequence: GECLGNVVSD ERRVSSSGGL QNAQFGIRRD GTLVTGYLSE EEVLDTENPF

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against NAGPA. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human NAGPA

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP47241-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for NAGPA

Target: NAGPA (N-Acetylglucosamine-1-phosphodiester alpha-N-Acetylglucosaminidase (NAGPA))

Alternative Name: NAGPA

Background:

Background Information: Hydrolases are transported to lysosomes after binding to mannose 6-phosphate receptors in the trans-Golgi network. This gene encodes the enzyme that catalyzes the second step in the formation of the mannose 6-phosphate recognition marker on lysosomal hydrolases. Commonly known as 'uncovering enzyme' or UCE, this enzyme removes N-acetyl-D-glucosamine (GlcNAc) residues from GlcNAc-alpha-P-mannose moieties and thereby produces the recognition marker. This reaction most likely occurs in the trans-Golgi network. This enzyme functions as a homotetramer of two disulfide-linked homodimers. In addition to having an N-terminal signal peptide, the protein's C-terminus contains multiple signals for trafficking it between lysosomes, the plasma membrane, and trans-Golgi network.

Gene Name: N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase

Alternative Symbols: UCE, APAA

Protein Name: N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase

Molecular Weight: 53kDa

Gene ID: 51172

NCBI Accession: NP_057340

UniProt: Q9UK23