HBEGF antibody (EGF Like Domain) (Biotin)

Catalog No. ABIN2451994

This Mouse Monoclonal antibody specifically detects HBEGF in WB, ELISA, IP, ICC and IF. It exhibits reactivity toward Human and has been mentioned in 4+ publications.

Quick Overview for HBEGF antibody (EGF Like Domain) (Biotin) (ABIN2451994)

Target: HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HBEGF antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: 4G10

Product Details anti-HBEGF Antibody (Biotin)

Binding Specificity: EGF Like Domain

Cross-Reactivity (Details): Does not react with mouse.

Characteristics: biotin/IgG = 7.5

Purification: Purified

Sterility: Sterile filtered

Immunogen: Recombinant human HB-EGF ectodomain expressed in SF21 cell

Isotype: IgG1

Application Details

Application Notes: 1) Western blotting: 0. 2~1 g/mL
2) Immunoprecipitation: 2 g/mL
3) Indirect immuno-fluorescence staining: 5~10 g/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol

Preservative: Azide free

Storage: -20 °C/-80 °C

Storage Comment: -20 C (For long term storage: -80 C)

References for anti-HBEGF antibody (Biotin) (ABIN2451994)

Miyamoto, Hirata, Yamazaki, Kageyama, Hasuwa, Mizushima, Tanaka, Yagi, Sonoda, Kai, Kanoh, Nakano, Mekada: "Heparin-binding EGF-like growth factor is a promising target for ovarian cancer therapy." in: Cancer research, Vol. 64, Issue 16, pp. 5720-7, (2004) (PubMed).

Prenzel, Zwick, Daub, Leserer, Abraham, Wallasch, Ullrich: "EGF receptor transactivation by G-protein-coupled receptors requires metalloproteinase cleavage of proHB-EGF." in: Nature, Vol. 402, Issue 6764, pp. 884-8, (2000) (PubMed).

Iwamoto, Higashiyama, Mitamura, Taniguchi, Klagsbrun, Mekada et al.: "Heparin-binding EGF-like growth factor, which acts as the diphtheria toxin receptor, forms a complex with membrane protein DRAP27/CD9, which up-regulates functional receptors and diphtheria toxin ..." in: The EMBO journal, Vol. 13, Issue 10, pp. 2322-30, (1994) (PubMed).

Higashiyama, Abraham, Miller, Fiddes, Klagsbrun: "A heparin-binding growth factor secreted by macrophage-like cells that is related to EGF." in: Science (New York, N.Y.), Vol. 251, Issue 4996, pp. 936-9, (1991) (PubMed).

Target Details for HBEGF

Target: HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))

Alternative Name: HB-EGF

Background: Background: Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is synthesized as a membrane-anchored precursor that is proteolytically cleaved to release the soluble mature growth factor, HB-EGF. The former functions as juxtacrine and the latter as paracrine growth factor. Soluble HB-EGF shows several forms in Western blotting with apparent molecular weights 19~27 kDa due to heterogeneous O-glycosylation and N-terminal truncation. HB-EGF activates EGFR and ErbB4 and promotes the development in many tissues. In human ProHB-EGF is the cellular receptor for diphtheria toxin. Non-toxic mutant of diphtheria toxin, CRM197, inhibits HB-EGF function. As HB-EGF level is elevated in most ovarian cancer, CRM197 is being tested as an anticancer drug. The hybridoma clone 4G10 was established and characterized in the laboratory of Prof. E. Mekada of Osaka University, who is a leading scientist in this field.

UniProt: Q99075

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway