NUP153 antibody (AA 610-1191)

Catalog No. ABIN2452062

Cited in 2+ publications. The Rat Monoclonal anti-NUP153 antibody (Clone R4C8) (ABIN2452062) specifically detects NUP153 in WB, ELISA, ICC and IF. The antibody is reactive with Rat samples.

Quick Overview for NUP153 antibody (AA 610-1191) (ABIN2452062)

Target: NUP153 (Nucleoporin 153kDa (NUP153))

Reactivity: Rat

Host: Rat

Clonality: Monoclonal

Conjugate: This NUP153 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: R4C8

Product Details anti-NUP153 Antibody

Binding Specificity: AA 610-1191

Specificity: Specific to human, mouse, rat and monkey Nup153 proteins.

Cross-Reactivity (Details): Not tested for other species

Characteristics: When injected into the nucleus,R4C8 accumulates into the nuclear pores of Hela cells. R4C8 works in immuno-cytochemistry very well.

Purification: Purified

Sterility: Sterile filtered

Immunogen: Recombinant GST-fused rat Nup153 (610-1191aa, Zn finger and FG repeats domain)

Isotype: IgG2a kappa

Application Details

Application Notes: 1. Western blotting: 160 kDa single band in Hela cell extract
2. Immunocytochemistry
3. ELISA
Other applications have not been tested.

Additional comments:
When injected into the nucleus, R4C8 accumulates into the nuclear pores of Hela cells.
R4C8 works in immuno-cytochemistry very well.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol

Preservative: Azide free

Storage: -20 °C

Storage Comment: Upon receipt aliquot and store at -20 C

References for anti-NUP153 antibody (ABIN2452062)

Lim, Fahrenkrog, Köser, Schwarz-Herion, Deng, Aebi: "Nanomechanical basis of selective gating by the nuclear pore complex." in: Science (New York, N.Y.), Vol. 318, Issue 5850, pp. 640-3, (2007) (PubMed).

Ullman, Shah, Powers, Forbes: "The nucleoporin nup153 plays a critical role in multiple types of nuclear export." in: Molecular biology of the cell, Vol. 10, Issue 3, pp. 649-64, (1999) (PubMed).

Target Details for NUP153

Target: NUP153 (Nucleoporin 153kDa (NUP153))

Alternative Name: Nup153

Background: The nuclear pore complex (NPC) regulates cargo transport between the cytoplasm and the nucleus. Nup (Nucleoporin) 153 is a large (153 kDa) O-linked glycoprotein which is a component of the basket structure located on the nucleoplasmic face of NPC. Nup153 plays a critical role in nuclear export of RNA and proteins. The antibody was purified from the serum-free cultured medium of the hybridoma under mild conditions by propriety chromatography processes.

UniProt: P49790

Pathways: Protein targeting to Nucleus