NUP98 antibody (GLFG Motif, N-Term)

Catalog No. ABIN2452064

The Mouse Monoclonal anti-NUP98 antibody has been validated for WB and IF. It is suitable to detect NUP98 in samples from Human, Mouse, Saccharomyces cerevisiae, Schizosaccharomyces pombe and Tetrahymena. There are 2+ publications available.

Quick Overview for NUP98 antibody (GLFG Motif, N-Term) (ABIN2452064)

Target: NUP98 (Nucleoporin 98kDa (NUP98))

Reactivity: Human, Mouse, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Tetrahymena

Host: Mouse

Clonality: Monoclonal

Conjugate: This NUP98 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 21A10

Product Details anti-NUP98 Antibody

Binding Specificity: GLFG Motif, N-Term

Cross-Reactivity (Details): Not tested in other species.

Purification: Purified

Sterility: Sterile filtered

Immunogen: Synthetic peptides containing conserved N-terminal sequence, GLFG, of Nup98 protein of Tetrahymena thermophila.

Isotype: IgG1 kappa

Application Details

Application Notes: 1) Western blotting: S. cerevisiae, S. pombe. Not suitable for human. Clone 13C2 is recommended for WB in human Tetrahymena and yeasts.
2) Most suitable for Immunofluorescence staining / Immunocytochemistry for human, mouse, Tetrahymena, S. cerevisiae, S. pombe.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol. Azide- and carrier protein-free.

Preservative: Azide free

Storage: -20 °C

Storage Comment: Upon arrival centrifuge briefly and store at -20 C.

References for anti-NUP98 antibody (ABIN2452064)

Iwamoto, Asakawa, Ohtsuki, Osakada, Koujin, Hiraoka, Haraguchi: "Monoclonal antibodies recognize gly-leu-phe-gly repeat of nucleoporin nup98 of tetrahymena, yeasts, and humans." in: Monoclonal antibodies in immunodiagnosis and immunotherapy, Vol. 32, Issue 2, pp. 81-90, (2013) (PubMed).

Iwamoto, Asakawa, Hiraoka, Haraguchi: "Nucleoporin Nup98: a gatekeeper in the eukaryotic kingdoms." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 15, Issue 7, pp. 661-9, (2010) (PubMed).

Target Details for NUP98

Target: NUP98 (Nucleoporin 98kDa (NUP98))

Alternative Name: Nup98

Background: Nucleoporin 98 (Nup98) is a component of nuclear pore complex (NPC), which is a large protein assembly embedded in the nuclear envelope and highly conserved in eukaryotes. It is localized on both nuclear and cytoplasmic side of NPC. This protein contains glycine-leucine-phenylalanine- glycine (GLFG) amino acid repeats and plays a critical role in nuclear trafficking. Nup98 also plays a specific role in the RNA export. In addition, Nup98 plays roles in several important biological events such as gene expression, mitotic checkpoint, and pathogenesis Nup98 gene is fused to a variety of partner genes in human myeloid and T-cell malignancies via chromosomal translocation. In ciliates, a unicellular organism having two functionally distinct nuclei, GLFG-Nup98 is present in one of the nuclei and a distinct Nup98 ortholog is present in the other nucleus, and these different Nup98s participate in a nucleus-selective transport mechanism.

UniProt: P52948

Pathways: Stem Cell Maintenance, Protein targeting to Nucleus, SARS-CoV-2 Protein Interactome