NUP98 antibody (AA 1-466)

Catalog No. ABIN2452066

Product Details anti-NUP98 Antibody

Target: NUP98 (Nucleoporin 98kDa (NUP98))

Binding Specificity: AA 1-466

Reactivity: Human, Mouse, Rat, Saccharomyces cerevisiae, Schizosaccharomyces pombe

Host: Rat

Clonality: Monoclonal

Conjugate: This NUP98 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Dot Blot (DB)

Cross-Reactivity (Details): This antibody does not react with Tetrahymena.
Other species have not been tested.

Purification: Purified

Sterility: Sterile filtered

Immunogen: Recombinant GST-fused human Nup98 (aa 1-466)

Clone: 2H10

Isotype: IgG2c kappa

Application Details

Application Notes: 1) Western blotting
2) Immunocytochemistry
3) ELISA
4) Dot blotting

Properties:
When injected into the cytoplasm, this antibody accumulates into the nuclear pores of HeLa cells and inhibits nuclear localization of endogenous Ran.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol. Azide- and carrier protein-free.

Preservative: Azide free

Storage: -20 °C

Storage Comment: Upon arrival centrifuge briefly and store at -20 C.

References for anti-NUP98 antibody (ABIN2452066)

Iwamoto, Asakawa, Ohtsuki, Osakada, Koujin, Hiraoka, Haraguchi: "Monoclonal antibodies recognize gly-leu-phe-gly repeat of nucleoporin nup98 of tetrahymena, yeasts, and humans." in: Monoclonal antibodies in immunodiagnosis and immunotherapy, Vol. 32, Issue 2, pp. 81-90, (2013) (PubMed).

Fukuhara, Ozaki, Shikata, Katahira, Yoneda, Ogino, Tachibana: "Specific monoclonal antibody against the nuclear pore complex protein, nup98." in: Hybridoma (2005), Vol. 24, Issue 5, pp. 244-7, (2005) (PubMed).

Target Details for NUP98

Target: NUP98 (Nucleoporin 98kDa (NUP98))

Alternative Name: Nup98 (NUP98 Products)

Synonyms: CG10198 antibody, CG10201 antibody, Dmel\\CG10198 antibody, Nup 96 antibody, Nup 98 antibody, Nup-98 antibody, Nup96 antibody, Nup98 antibody, Nup98-Nup96 antibody, Nup98/96 antibody, l(3)95BCd antibody, nup145 antibody, nup98-96 antibody, GB12688 antibody, NUP98 antibody, 4732457F17 antibody, AI849286 antibody, im:7151238 antibody, zgc:113968 antibody, zgc:63593 antibody, ADIR2 antibody, NUP196 antibody, NUP96 antibody, Nucleoporin 98-96kD antibody, nuclear pore complex protein Nup98-Nup96 antibody, nucleoporin 98 antibody, nucleoporin 98kDa antibody, nucleoporin 98 L homeolog antibody, Nup98-96 antibody, LOC408335 antibody, NUP98 antibody, LOC587580 antibody, Nup98 antibody, nup98 antibody, nup98.L antibody

Background: Nucleoporin 98 (Nup98) is a component of nuclear pore complex (NPC), which is a large protein assembly embedded in the nuclear envelope. It is localized on both nuclear and cytoplasmic side of NPC. This protein contains glycine-leucine-phenylalanine-glycine (GLFG) amino acid repeats and plays a critical role in nuclear trafficking. Nup98 plays a specific role in the RNA export. Nup98 gene is fused to a variety of partner genes in human myeloid and T-cell malignancies via chromosomal translocation. This hybridoma has been established by Prof. T. Tachibana's Lab. at Osaka City Univ.

UniProt: Q9HDC8

Pathways: Stem Cell Maintenance, Protein targeting to Nucleus, SARS-CoV-2 Protein Interactome