RMI1 antibody

Catalog No. ABIN2459011

This Rabbit Polyclonal antibody specifically detects RMI1 in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for RMI1 antibody (ABIN2459011)

Target: RMI1 (Homolog of Yeast RecQ-mediated Genome Instability 1 (RMI1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RMI1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RMI1 Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RMI1.

Application Details

Application Notes: RMI1 antibody can be used for detection of RMI1 by ELISA at 1:1562500. RMI1 antibody can be used for detection of RMI1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store RMI1 antibody at -20 °C.

Target Details for RMI1

Target: RMI1 (Homolog of Yeast RecQ-mediated Genome Instability 1 (RMI1))

Alternative Name: RMI1

Background: RMI1 is an essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. RMI1 promotes TOP3A binding to double Holliday junctions (DHJ) and hence stimulates TOP3A-mediated dissolution. RMI1 is required for BLM phosphorylation during mitosis. Within the BLM complex, RMI1 is required for BLM and TOP3A stability.RMI1 is a component of protein complexes that limit DNA crossover formation via the dissolution of double Holliday junctions (Raynard et al., 2006 [PubMed 16595695]).

Molecular Weight: 70 kDa

Gene ID: 80010

NCBI Accession: NP_079221

UniProt: Q9H9A7

Pathways: DNA Damage Repair