Klotho beta antibody

Catalog No. ABIN2459659

This Rabbit Polyclonal antibody specifically detects Klotho beta in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Klotho beta antibody (ABIN2459659)

Target: Klotho beta (KLB)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Klotho beta antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Klotho beta Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human KLB.

Application Details

Application Notes: KLB antibody can be used for detection of KLB by ELISA at 1:12500. KLB antibody can be used for detection of KLB by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store KLB antibody at -20 °C.

Target Details for Klotho beta

Target: Klotho beta (KLB)

Alternative Name: KLB

Background: KLB is a single-pass type III membrane protein. It contributes to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis. KLB is probably inactive as a glycosidase. It increases the ability of FGFR1 and FGFR4 to bind FGF21.

Molecular Weight: 120 kDa

Gene ID: 152831

NCBI Accession: NP_783864

UniProt: Q86Z14

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Growth Factor Binding