K-RAS antibody

Catalog No. ABIN2459898

This anti-K-RAS antibody is a Rabbit Polyclonal antibody detecting K-RAS in WB and ELISA. Suitable for Human, Rat, Mouse and Drosophila melanogaster.

Quick Overview for K-RAS antibody (ABIN2459898)

Target: K-RAS (KRAS) (GTPase Kras (KRAS))

Reactivity: Human, Rat, Mouse, Drosophila melanogaster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This K-RAS antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-K-RAS Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human KRAS.

Application Details

Application Notes: KRAS antibody can be used for detection of KRAS by ELISA at 1:62500. KRAS antibody can be used for detection of KRAS by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store KRAS antibody at -20 °C.

Target Details for K-RAS

Target: K-RAS (KRAS) (GTPase Kras (KRAS))

Alternative Name: KRAS

Target Type: Viral Protein

Background: KRAS is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.

Molecular Weight: 22 kDa

Gene ID: 3845

NCBI Accession: NP_203524

UniProt: P01111