POU1F1 antibody

Catalog No. ABIN2460215

The Rabbit Polyclonal anti-POU1F1 antibody is suitable to detect POU1F1 in samples from Human, Mouse, Rat and Dog. It has been validated for WB, ELISA and IHC.

Quick Overview for POU1F1 antibody (ABIN2460215)

Target: POU1F1 (POU Class 1 Homeobox 1 (POU1F1))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POU1F1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-POU1F1 Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POU1F1.

Application Details

Application Notes: POU1F1 antibody can be used for detection of POU1F1 by ELISA at 1:12500. POU1F1 antibody can be used for detection of POU1F1 by western blot at 1.0-10.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store POU1F1 antibody at -20 °C.

Target Details for POU1F1

Target: POU1F1 (POU Class 1 Homeobox 1 (POU1F1))

Alternative Name: POU1F1

Background: PIT1 is a pituitary-specific transcription factor responsible for pituitary development and hormone expression in mammals and is a member of the POU family of transcription factors that regulate mammalian development. The POU family is so named because the first 3 members identified were PIT1 and OCT1 of mammals, and Unc-86 of C. elegans. PIT1 contains 2 protein domains, termed POU-specific and POU-homeo, which are both necessary for high affinity DNA binding on genes encoding growth hormone and prolactin. PIT1 is also important for regulation of the genes encoding prolactin and thyroid-stimulating hormone beta subunit by thyrotropin-releasing hormone and cyclic AMP.

Molecular Weight: 33 kDa

Gene ID: 5449

NCBI Accession: NP_000297

UniProt: P28069

Pathways: Regulation of Carbohydrate Metabolic Process