SMAD5 antibody

Catalog No. ABIN2460351

The Rabbit Polyclonal anti-SMAD5 antibody (ABIN2460351) specifically detects SMAD5 in WB and ELISA. The antibody is reactive with Human, Mouse, Rat and Dog samples.

Quick Overview for SMAD5 antibody (ABIN2460351)

Target: SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-SMAD5 Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMAD5.

Application Details

Application Notes: SMAD5 antibody can be used for detection of SMAD5 by ELISA at 1:62500. SMAD5 antibody can be used for detection of SMAD5 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store SMAD5 antibody at -20 °C.

Target Details for SMAD5

Target: SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))

Alternative Name: SMAD5

Background: SMAD5 undergoes copy number gain and increased expression, rather than loss of expression, and therefore does not act as a tumor-suppressor gene in hepatocellular carcinoma. Up-regulated Smad5 mediates apoptosis of gastric epithelial cells induced by Helicobacter pylori infection.

Molecular Weight: 51 kDa

Gene ID: 4090

NCBI Accession: NP_005894

UniProt: Q99717