PAWR antibody

Catalog No. ABIN2460368

Product Details anti-PAWR Antibody

Target: PAWR (PRKC, Apoptosis, WT1, Regulator (PAWR))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAWR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PAWR.

Application Details

Application Notes: PAWR antibody can be used for detection of PAWR by ELISA at 1:62500. PAWR antibody can be used for detection of PAWR by western blot at 0.2-5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store PAWR antibody at -20 °C.

Target Details for PAWR

Target: PAWR (PRKC, Apoptosis, WT1, Regulator (PAWR))

Alternative Name: PAWR (PAWR Products)

Background: The tumor suppressor WT1 represses and activates transcription. Anti-Prostate Apoptosis Response Protein Par-4 (PAWR) is a WT1-interacting protein that itself functions as a transcriptional repressor. It contains a putative leucine zipper domain which interacts with the zinc finger DNA binding domain of WT1. This protein is specifically upregulated during apoptosis of prostate cells.

Molecular Weight: 37 kDa

Gene ID: 5074

NCBI Accession: NP_002574

UniProt: O75796