HNF1B antibody

Catalog No. ABIN2460425

The Rabbit Polyclonal anti-HNF1B antibody has been validated for WB and ELISA. It is suitable to detect HNF1B in samples from Human, Rat, Mouse, Zebrafish (Danio rerio) and Dog.

Quick Overview for HNF1B antibody (ABIN2460425)

Target: HNF1B (HNF1 Homeobox B (HNF1B))

Reactivity: Human, Rat, Mouse, Zebrafish (Danio rerio), Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HNF1B antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-HNF1B Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TCF2.

Application Details

Application Notes: TCF2 antibody can be used for detection of TCF2 by ELISA at 1:62500. TCF2 antibody can be used for detection of TCF2 by western blot at 0.1-2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store TCF2 antibody at -20 °C.

Target Details for HNF1B

Target: HNF1B (HNF1 Homeobox B (HNF1B))

Alternative Name: TCF2

Background: Hepatocyte Nuclear Factor 1-. (HNF-1., Homeoprotein LFB3, Transcription factor 2, TCF2, Variant hepatic nuclear factor ) is a liver-specific factor of the homeobox-containing basic helix-turn-helix family. The TCF2 protein is believed to form heterodimers with another liver-specific member of this transcription factor family, TCF1, depending on the TCF2 isoform, the result may be to activate or inhibit transcription of target genes. Mutation of TCF2 that disrupts normal function has been identified as the cause of MODY5 (Maturity-Onset of Diabetes, Type 5). A third human transcript variant is believed to exist based on such a variant in the rat: however, to date such an mRNA species has not been isolated.

Molecular Weight: 45 kDa, 61 kDa

Gene ID: 6928

NCBI Accession: NP_006472

UniProt: P35680

Pathways: Hormone Transport, Stem Cell Maintenance, Tube Formation