POLR2B antibody

Catalog No. ABIN2460473

The Rabbit Polyclonal anti-POLR2B antibody has been validated for WB and ELISA. It is suitable to detect POLR2B in samples from Human, Mouse, Rat and Dog.

Quick Overview for POLR2B antibody (ABIN2460473)

Target: POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2B antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-POLR2B Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POLR2B.

Application Details

Application Notes: POLR2B antibody can be used for detection of POLR2B by ELISA at 1:1562500. POLR2B antibody can be used for detection of POLR2B by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store POLR2B antibody at -20 °C.

Target Details for POLR2B

Target: POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))

Alternative Name: POLR2B

Background: POLR2B encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA.

Molecular Weight: 134 kDa

Gene ID: 5431

NCBI Accession: NP_000929

UniProt: P30876

Pathways: Regulatory RNA Pathways, DNA Damage Repair