KAT2B antibody

Catalog No. ABIN2460477

The Rabbit Polyclonal anti-KAT2B antibody (ABIN2460477) specifically detects KAT2B in WB and ELISA. The antibody is reactive with Human, Mouse and Dog samples.

Quick Overview for KAT2B antibody (ABIN2460477)

Target: KAT2B (K(lysine) Acetyltransferase 2B (KAT2B))

Reactivity: Human, Mouse, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KAT2B antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-KAT2B Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PCAF.

Application Details

Application Notes: PCAF antibody can be used for detection of PCAF by ELISA at 1:62500. PCAF antibody can be used for detection of PCAF by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store PCAF antibody at -20 °C.

Target Details for KAT2B

Target: KAT2B (K(lysine) Acetyltransferase 2B (KAT2B))

Alternative Name: PCAF

Background: CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. PCAF associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation.CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation.

Molecular Weight: 93 kDa

Gene ID: 8850

NCBI Accession: NP_003875

UniProt: Q92831

Pathways: p53 Signaling, Regulation of Carbohydrate Metabolic Process