The Rabbit Polyclonal anti-HIF1AN antibody has been validated for WB and ELISA. It is suitable to detect HIF1AN in samples from Human, Mouse, Rat and Dog.
HIF1AN antibody can be used for detection of HIF1AN by ELISA at 1:1562500. HIF1AN antibody can be used for detection of HIF1AN by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Concentration
1 mg/mL
Buffer
Antibody is lyophilized in PBS buffer with 2 % sucrose.
Handling Advice
As with any antibody avoid repeat freeze-thaw cycles.
Storage
4 °C/-20 °C
Storage Comment
For short periods of storage (days) store at 4 °C. For longer periods of storage, store HIF1AN antibody at -20 °C.
HIF1AN is a co-repressor that interacts with hypoxia-inducible factor 1 (HIF-1) alpha and the von Hippel-Lindau tumor suppressor protein to mediate repression of HIF-1 transcriptional activity.