ARID4A antibody

Catalog No. ABIN2460649

This Rabbit Polyclonal antibody specifically detects ARID4A in WB and ELISA. It exhibits reactivity toward Human and Dog.

Quick Overview for ARID4A antibody (ABIN2460649)

Target: ARID4A (AT Rich Interactive Domain 4A (RBP1-Like) (ARID4A))

Reactivity: Human, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARID4A antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ARID4A Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ARID4A.

Application Details

Application Notes: ARID4A antibody can be used for detection of ARID4A by ELISA at 1:62500. ARID4A antibody can be used for detection of ARID4A by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store ARID4A antibody at -20 °C.

Target Details for ARID4A

Target: ARID4A (AT Rich Interactive Domain 4A (RBP1-Like) (ARID4A))

Alternative Name: ARID4A

Background: ARID4A encodes a ubiquitously expressed nuclear protein. It binds directly, with several other proteins, to retinoblastoma protein (pRB) which regulates cell proliferation. pRB represses transcription by recruiting the encoded protein. This protein, in turn, serves as a bridging molecule to recruit HDACs and, in addition, provides a second HDAC-independent repression function. The encoded protein possesses transcriptional repression activity. Multiple alternatively spliced transcripts have been observed for this gene, although not all transcript variants have been fully described.

Molecular Weight: 143 kDa

Gene ID: 5926

NCBI Accession: NP_002883

UniProt: P29374