STAT4 antibody

Catalog No. ABIN2460707

The Rabbit Polyclonal anti-STAT4 antibody is suitable to detect STAT4 in samples from Human and Dog. It has been validated for WB, IHC and ELISA.

Quick Overview for STAT4 antibody (ABIN2460707)

Target: STAT4 (Signal Transducer and Activator of Transcription 4 (STAT4))

Reactivity: Human, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STAT4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-STAT4 Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human STAT4.

Application Details

Application Notes: STAT4 antibody can be used for detection of STAT4 by ELISA at 1:62500. STAT4 antibody can be used for detection of STAT4 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store STAT4 antibody at -20 °C.

Target Details for STAT4

Target: STAT4 (Signal Transducer and Activator of Transcription 4 (STAT4))

Alternative Name: STAT4

Background: STAT4 is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells.

Molecular Weight: 86 kDa

Gene ID: 6775

NCBI Accession: NP_003142

UniProt: Q14765

Pathways: JAK-STAT Signaling