MED17 antibody

Catalog No. ABIN2460890

The Rabbit Polyclonal anti-MED17 antibody is suitable to detect MED17 in samples from Human, Rat and Dog. It has been validated for WB, ELISA and IHC.

Quick Overview for MED17 antibody (ABIN2460890)

Target: MED17 (Mediator Complex Subunit 17 (MED17))

Reactivity: Human, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED17 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-MED17 Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CRSP6.

Application Details

Application Notes: CRSP6 antibody can be used for detection of CRSP6 by ELISA at 1:12500. CRSP6 antibody can be used for detection of CRSP6 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store CRSP6 antibody at -20 °C.

Target Details for MED17

Target: MED17 (Mediator Complex Subunit 17 (MED17))

Alternative Name: CRSP6

Background: The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by CRSP6 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.

Molecular Weight: 73 kDa

Gene ID: 9440

NCBI Accession: NP_004259

UniProt: Q9NVC6

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha