Aspartate beta Hydroxylase antibody

Catalog No. ABIN2460891

The Rabbit Polyclonal anti-Aspartate beta Hydroxylase antibody (ABIN2460891) specifically detects Aspartate beta Hydroxylase in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Aspartate beta Hydroxylase antibody (ABIN2460891)

Target: Aspartate beta Hydroxylase (ASPH) (Aspartate beta-Hydroxylase (ASPH))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aspartate beta Hydroxylase antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Aspartate beta Hydroxylase Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ASPH.

Application Details

Application Notes: ASPH antibody can be used for detection of ASPH by ELISA at 1:312500. ASPH antibody can be used for detection of ASPH by western blot at 5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store ASPH antibody at -20 °C.

Target Details for Aspartate beta Hydroxylase

Target: Aspartate beta Hydroxylase (ASPH) (Aspartate beta-Hydroxylase (ASPH))

Alternative Name: ASPH

Background: ASPH is thought to play an important role in calcium homeostasis. Alternative splicing of this gene results in five transcript variants which vary in protein translation, the coding of catalytic domains, and tissue expression. Variation among these transcripts impacts their functions which involve roles in the calcium storage and release process in the endoplasmic and sarcoplasmic reticulum as well as hydroxylation of aspartic acid and asparagine in epidermal growth factor-like domains of various proteins.

Molecular Weight: 86 kDa

Gene ID: 444

NCBI Accession: NP_004309

UniProt: Q12797

Pathways: Positive Regulation of Endopeptidase Activity