GJB2 antibody

Catalog No. ABIN2461384

This Rabbit Polyclonal antibody specifically detects GJB2 in WB and ELISA. It exhibits reactivity toward Human and Dog.

Quick Overview for GJB2 antibody (ABIN2461384)

Target: GJB2 (Gap Junction Protein, beta 2, 26kDa (GJB2))

Reactivity: Human, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GJB2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-GJB2 Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GJB2.

Application Details

Application Notes: GJB2 antibody can be used for detection of GJB2 by ELISA at 1:62500. GJB2 antibody can be used for detection of GJB2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store GJB2 antibody at -20 °C.

Target Details for GJB2

Target: GJB2 (Gap Junction Protein, beta 2, 26kDa (GJB2))

Alternative Name: GJB2

Background: Gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell channels. Proteins, called connexins, purified from fractions of enriched gap junctions from different tissues differ. The connexins are designated by their molecular mass. Another system of nomenclature divides gap junction proteins into 2 categories, alpha and beta, according to sequence similarities at the nucleotide and amino acid levels. For example, CX43 (MIM 121014) is designated alpha-1 gap junction protein, whereas CX32 (GJB1, MIM 304040) and CX26 are called beta-1 and beta-2 gap junction proteins, respectively. This nomenclature emphasizes that CX32 and CX26 are more homologous to each other than either of them is to CX43.

Molecular Weight: 25 kDa

Gene ID: 2706

NCBI Accession: NP_003995

UniProt: P29033

Pathways: Sensory Perception of Sound, Cell-Cell Junction Organization