Glypican 3 antibody
Quick Overview for Glypican 3 antibody (ABIN2461568)
Target
See all Glypican 3 (GPC3) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Purification
- Antibody is purified by protein A chromatography method.
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Immunogen
- Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GPC3.
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Application Notes
- GPC3 antibody can be used for detection of GPC3 by ELISA at 1:1562500. GPC3 antibody can be used for detection of GPC3 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.
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Concentration
- 1 mg/mL
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Buffer
- Antibody is lyophilized in PBS buffer with 2 % sucrose.
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Handling Advice
- As with any antibody avoid repeat freeze-thaw cycles.
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Storage
- 4 °C/-20 °C
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Storage Comment
- For short periods of storage (days) store at 4 °C. For longer periods of storage, store GPC3 antibody at -20 °C.
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- Glypican 3 (GPC3)
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Alternative Name
- GPC3
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Background
- Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome.
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Molecular Weight
- 64 kDa
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Gene ID
- 2719
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NCBI Accession
- NP_004475
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UniProt
- P51654
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Pathways
- Glycosaminoglycan Metabolic Process
Target
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