EDF1 antibody

Catalog No. ABIN2461586

The Rabbit Polyclonal anti-EDF1 antibody has been validated for WB and ELISA. It is suitable to detect EDF1 in samples from Human, Mouse, Rat and Dog.

Quick Overview for EDF1 antibody (ABIN2461586)

Target: EDF1 (Endothelial Differentiation Related Factor 1 (EDF1))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EDF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-EDF1 Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EDF1.

Application Details

Application Notes: EDF1 antibody can be used for detection of EDF1 by ELISA at 1:312500. EDF1 antibody can be used for detection of EDF1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store EDF1 antibody at -20 °C.

Target Details for EDF1

Target: EDF1 (Endothelial Differentiation Related Factor 1 (EDF1))

Alternative Name: EDF1

Background: EDF1 encodes a protein that may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the transcription of genes involved in endothelial differentiation. This protein has also been found to act as a transcriptional coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Two alternatively spliced transcripts which encode distinct proteins have been found for this gene.

Molecular Weight: 16 kDa, 15 kDa

Gene ID: 8721

NCBI Accession: NP_003783

UniProt: O60869