PRMT5 antibody
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- Target See all PRMT5 Antibodies
- PRMT5 (Protein Arginine Methyltransferase 5 (PRMT5))
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Reactivity
- Human, Mouse, Rat, Dog
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PRMT5 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- Purification
- Antibody is purified by protein A chromatography method.
- Immunogen
- Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PRMT5.
- Top Product
- Discover our top product PRMT5 Primary Antibody
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- Application Notes
- PRMT5 antibody can be used for detection of PRMT5 by ELISA at 1:62500. PRMT5 antibody can be used for detection of PRMT5 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.
- Concentration
- 1 mg/mL
- Buffer
- Antibody is lyophilized in PBS buffer with 2 % sucrose.
- Handling Advice
- As with any antibody avoid repeat freeze-thaw cycles.
- Storage
- 4 °C/-20 °C
- Storage Comment
- For short periods of storage (days) store at 4 °C. For longer periods of storage, store PRMT5 antibody at -20 °C.
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- Target
- PRMT5 (Protein Arginine Methyltransferase 5 (PRMT5))
- Alternative Name
- PRMT5 (PRMT5 Products)
- Synonyms
- HRMT1L5 antibody, IBP72 antibody, JBP1 antibody, SKB1 antibody, SKB1Hs antibody, Jbp1 antibody, Skb1 antibody, si:zc14a17.2 antibody, skb1 antibody, wu:fb95f10 antibody, zgc:110669 antibody, hsl7 antibody, DDBDRAFT_0187422 antibody, DDBDRAFT_0235403 antibody, DDB_0187422 antibody, DDB_0235403 antibody, NV18830 antibody, jbp1 antibody, ibp72 antibody, skb1hs antibody, hrmt1l5 antibody, protein arginine methyltransferase 5 antibody, protein arginine N-methyltransferase 5 antibody, protein arginine methyltransferase 5 L homeolog antibody, putative arginine N-methyltransferase, type II antibody, Skb1 family protein antibody, Protein arginine N-methyltransferase 5 antibody, PRMT5 antibody, Prmt5 antibody, prmt5 antibody, prmt5.L antibody, LOC100115766 antibody, prmt-5 antibody
- Background
- PRMT5 methylates specific arginine residues in the small nuclear ribonucleoproteins Sm D1 and Sm D3 to monomethylarginine and to symmetrical dimethylarginines (sDMAs). It methylates SUPT5H. PRMT5 plays a role in the assembly of snRNP core particles and may play a role in cytokine-activated transduction pathways. It negatively regulates cyclin E1 promoter activity and cellular proliferation and May regulate the SUPT5H transcriptional elongation properties. It may be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. PRMT5 methylates histone H2A/H4 'Arg-3' during germ cell development and methylates histone H3 'Arg-8', which may repress transcription.
- Molecular Weight
- 68 kDa, 73 kDa
- Gene ID
- 10419
- NCBI Accession
- NP_001034708
- UniProt
- O14744
- Pathways
- Chromatin Binding, Regulation of Muscle Cell Differentiation, Ribonucleoprotein Complex Subunit Organization, Skeletal Muscle Fiber Development
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