PCBP2 antibody

Catalog No. ABIN2462179

Product Details anti-PCBP2 Antibody

Target: PCBP2 (Poly(rC) Binding Protein 2 (PCBP2))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCBP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PCBP2.

Application Details

Application Notes: PCBP2 antibody can be used for detection of PCBP2 by ELISA at 1:312500. PCBP2 antibody can be used for detection of PCBP2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store PCBP2 antibody at -20 °C.

Target Details for PCBP2

Target: PCBP2 (Poly(rC) Binding Protein 2 (PCBP2))

Alternative Name: PCBP2 (PCBP2 Products)

Synonyms: HNRNPE2 antibody, HNRPE2 antibody, hnRNP-E2 antibody, AW412548 antibody, Hnrpx antibody, alphaCP-2 antibody, PCBP3 antibody, fb36h02 antibody, wu:fb36h02 antibody, zgc:55964 antibody, PCBP2 antibody, poly(rC) binding protein 2 antibody, poly(rC) binding protein 2 L homeolog antibody, PCBP2 antibody, Pcbp2 antibody, pcbp2 antibody, pcbp2.L antibody

Background: PCBP2 appears to be multifunctional. It along with PCBP-1 and hnRNPK corresponds to the major cellular poly (rC)-binding proteins. This protein together with PCBP-1 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This gene and PCBP-1 has paralogues PCBP3 and PCBP4 which is thought to arose as a result of duplication events of entire genes.The protein encoded by this gene appears to be multifunctional. It along with PCBP-1 and hnRNPK corresponds to the major cellular poly (rC)-binding proteins. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-1 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1 intronless gene which has similar functions. This gene and PCBP-1 has paralogues PCBP3 and PCBP4 which is thought to arose as a result of duplication events of entire genes. It also has two processed pseudogenes PCBP2P1 and PCBP2P2. There are presently two alternatively spliced transcript variants described for this gene.

Molecular Weight: 40 kDa, 38 kDa

Gene ID: 5094

NCBI Accession: NP_005007

UniProt: Q6IPF4