MSH2 antibody

Catalog No. ABIN2462390

The Rabbit Polyclonal anti-MSH2 antibody has been validated for WB and ELISA. It is suitable to detect MSH2 in samples from Human, Mouse, Dog and Zebrafish (Danio rerio).

Quick Overview for MSH2 antibody (ABIN2462390)

Target: MSH2 (Mismatch Repair Protein 2 (MSH2))

Reactivity: Human, Mouse, Dog, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MSH2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MSH2 Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MSH2.

Application Details

Application Notes: MSH2 antibody can be used for detection of MSH2 by ELISA at 1:62500. MSH2 antibody can be used for detection of MSH2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store MSH2 antibody at -20 °C.

Target Details for MSH2

Target: MSH2 (Mismatch Repair Protein 2 (MSH2))

Alternative Name: MSH2

Background: MSH2 was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC.

Molecular Weight: 64 kDa, 105 kDa

Gene ID: 4436

UniProt: P43246

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response