BHMT antibody

Catalog No. ABIN2462417

The Rabbit Polyclonal anti-BHMT antibody is suitable to detect BHMT in samples from Human, Mouse, Rat and Dog. It has been validated for WB, IHC and ELISA.

Quick Overview for BHMT antibody (ABIN2462417)

Target: BHMT (Betaine--Homocysteine S-Methyltransferase (BHMT))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BHMT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-BHMT Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human BHMT.

Application Details

Application Notes: BHMT antibody can be used for detection of BHMT by ELISA at 1:312500. BHMT antibody can be used for detection of BHMT by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store BHMT antibody at -20 °C.

Target Details for BHMT

Target: BHMT (Betaine--Homocysteine S-Methyltransferase (BHMT))

Alternative Name: BHMT

Background: BHMT is a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in its gene could lead to hyperhomocyst (e)inemia, but such a defect has not yet been observed.Betaine-homocysteine methyltransferase is a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in BHMT could lead to hyperhomocyst (e)inemia,but such a defect has not yet been observed.

Molecular Weight: 45 kDa

Gene ID: 635

NCBI Accession: NP_001704

UniProt: Q93088

Pathways: Methionine Biosynthetic Process