RFC3 antibody

Catalog No. ABIN2462934

Product Details anti-RFC3 Antibody

Target: RFC3 (Replication Factor C (Activator 1) 3, 38kDa (RFC3))

Reactivity: Human, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFC3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RFC3.

Application Details

Application Notes: RFC3 antibody can be used for detection of RFC3 by ELISA at 1:62500. RFC3 antibody can be used for detection of RFC3 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store RFC3 antibody at -20 °C.

Target Details for RFC3

Target: RFC3 (Replication Factor C (Activator 1) 3, 38kDa (RFC3))

Alternative Name: RFC3 (RFC3 Products)

Synonyms: CG5313 antibody, DRFC antibody, DmRFC3 antibody, Dmel\\CG5313 antibody, Rfc3 antibody, 21.m02902 antibody, 2810416I22Rik antibody, 38kDa antibody, AU022547 antibody, Recc3 antibody, cb275 antibody, RFC38 antibody, Replication factor C subunit 3 antibody, replication factor C3 antibody, replication factor C subunit 3 antibody, replication factor C 38 kDa subunit antibody, DNA replication factor C complex subunit Rfc3 antibody, replication factor C3, putative antibody, replication factor C (activator 1) 3 antibody, replication factor C subunit 3 L homeolog antibody, RfC3 antibody, PF14_0601 antibody, Chro.30359 antibody, PB000006.01.0 antibody, PC000345.04.0 antibody, TP04_0380 antibody, Tb09.211.3310 antibody, PY04255 antibody, PVX_117300 antibody, BBOV_IV003080 antibody, SJAG_02182 antibody, PKH_124240 antibody, rfc3 antibody, Rfc3 antibody, RFC3 antibody, rfc3.L antibody

Background: The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. RFC3 is the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits.The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 38 kD subunit. This subunit is essential for the interaction between the 140 kD subunit and the core complex that consists of the 36, 37, and 40 kD subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Molecular Weight: 40 kDa

Gene ID: 5983

NCBI Accession: NP_002906

UniProt: P40938

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA