DPH1 antibody

Catalog No. ABIN2463486

This anti-DPH1 antibody is a Rabbit Polyclonal antibody detecting DPH1 in WB and ELISA. Suitable for Human and Mouse.

Quick Overview for DPH1 antibody (ABIN2463486)

Target: DPH1 (DPH1 Homolog (DPH1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DPH1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-DPH1 Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DPH1.

Application Details

Application Notes: DPH1 antibody can be used for detection of DPH1 by ELISA at 1:312500. DPH1 antibody can be used for detection of DPH1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store DPH1 antibody at -20 °C.

Target Details for DPH1

Target: DPH1 (DPH1 Homolog (DPH1))

Alternative Name: DPH1

Background: Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2, MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH1 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]). Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2, MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH1 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).

Molecular Weight: 49 kDa

Gene ID: 1801

NCBI Accession: NP_001374

UniProt: Q9BZG8