DPH1 antibody (N-Term)

Catalog No. ABIN2784707

This Rabbit Polyclonal antibody specifically detects DPH1 in WB and IHC. It exhibits reactivity toward Human, Rat, Mouse, Dog, Guinea Pig, Horse, Rabbit, Cow, Saccharomyces cerevisiae and Zebrafish (Danio rerio).

Quick Overview for DPH1 antibody (N-Term) (ABIN2784707)

Target: DPH1 (DPH1 Homolog (DPH1))

Reactivity: Human, Rat, Mouse, Dog, Guinea Pig, Horse, Rabbit, Cow, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DPH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-DPH1 Antibody

Binding Specificity: N-Term

Purpose: DPH1 Antibody - N-terminal region

Sequence: NQIPPEILKN PQLQAAIRVL PSNYNFEIPK TIWRIQQAQA KKVALQMPEG

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 92%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against DPH1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DPH1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP51955-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for DPH1

Target: DPH1 (DPH1 Homolog (DPH1))

Alternative Name: DPH1

Background:

Background Information: Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2, MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH1 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).

Gene Name: DPH1 homolog (S. cerevisiae)

Alternative Symbols: DPH2L, OVCA1, DEDSSH, DPH2L1

Protein Name: Diphthamide biosynthesis protein 1

Molecular Weight: 49kDa

Gene ID: 1801

NCBI Accession: NP_001374

UniProt: Q9BZG8