CDK9 antibody

Catalog No. ABIN2463669

This anti-CDK9 antibody is a Rabbit Polyclonal antibody detecting CDK9 in WB and ELISA. Suitable for Human and Dog.

Quick Overview for CDK9 antibody (ABIN2463669)

Target: CDK9 (Cyclin-Dependent Kinase 9 (CDK9))

Reactivity: Human, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CDK9 Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CDK9.

Application Details

Application Notes: CDK9 antibody can be used for detection of CDK9 by ELISA at 1:312500. CDK9 antibody can be used for detection of CDK9 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store CDK9 antibody at -20 °C.

Target Details for CDK9

Target: CDK9 (Cyclin-Dependent Kinase 9 (CDK9))

Alternative Name: CDK9

Background: CDK9 is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS.The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS.

Molecular Weight: 43 kDa

Gene ID: 1025

NCBI Accession: NP_001252

UniProt: P50750

Pathways: Cell Division Cycle