Cyclin D3 antibody

Catalog No. ABIN2463678

This anti-Cyclin D3 antibody is a Rabbit Polyclonal antibody detecting Cyclin D3 in WB, ELISA and IHC. Suitable for Human, Mouse, Rat and Dog.

Quick Overview for Cyclin D3 antibody (ABIN2463678)

Target: Cyclin D3 (CCND3)

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin D3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-Cyclin D3 Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CCND3.

Application Details

Application Notes: CCND3 antibody can be used for detection of CCND3 by ELISA at 1:62500. CCND3 antibody can be used for detection of CCND3 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store CCND3 antibody at -20 °C.

Target Details for Cyclin D3

Target: Cyclin D3 (CCND3)

Alternative Name: CCND3

Background: CCND3 encodes a protein that belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation.

Molecular Weight: 33 kDa

Gene ID: 896

NCBI Accession: NP_001751

UniProt: P30281

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Glycosaminoglycan Metabolic Process