TBP antibody

Catalog No. ABIN2463874

This Rabbit Polyclonal antibody specifically detects TBP in WB and ELISA. It exhibits reactivity toward Human, Mouse, Rat, Zebrafish (Danio rerio) and Dog.

Quick Overview for TBP antibody (ABIN2463874)

Target: TBP (TATA Box Binding Protein (TBP))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TBP antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-TBP Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TBP.

Application Details

Application Notes: TBP antibody can be used for detection of TBP by ELISA at 1:62500. TBP antibody can be used for detection of TBP by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store TBP antibody at -20 °C.

Target Details for TBP

Target: TBP (TATA Box Binding Protein (TBP))

Alternative Name: TBP

Background: Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminal. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. Mutations that expand the number of CAG repeats encoding this polyglutamine tract, and thus increase the length of the polyglutamine string, are associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease.

Molecular Weight: 38 kDa

Gene ID: 6908

NCBI Accession: NP_003185

UniProt: P20226

Pathways: WNT Signaling